Purpose: study of mental health and lifestyle of midwives plays a significant role in the health of their social and individual lives. In this regard and with respect to that midwives are at risk of burnout, determining factors that can endanger the health of midwives seems very necessary. Researcher purpose of this research is to compare the mental health and working life of midwives in the health area with midwives working in the field of treatment of health centers at Shahid Beheshti University of Mental Sciences. Research method: the present research was causal – comparative. The statistical community consisted of all midwives working in the health and treatment sector in Shahid Beheshti University of Mental Sciences. from introduced community was selected an example of 300 people (each group has 150 people) according to Cochran sampling formula by available sampling method and the general health questionnaire (12-GHQ) and health promoting lifestyle questionnaire were evaluated by using demographic profile questionnaire. Then the collected data were analyzed by using statistical analysis of variance and T test utilizing the software spss-16.Findings: The mental health average in the treatment group (15.86+-6.51) was more than average in the health group (15.26+-6.71) also average lifestyle status in health group (123.39+-37.39) was more than average of treatment group (111.87+-38.92).Conclusion: with respect to T test results in mental health was not observed a meaningful relationship among two groups of the treatment and health. But in the field of the lifestyle was observed a meaningful relationship between treatment and health groups. Therefore, health centers can increase the work commitment and the growth and dynamics of health services with increasing mental health level and improving the lifestyle of midwives.