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2015 Volume 6 Issue 3

Cold active alpha amylase from a psychrophilic bacterial isolate


Abstract
In the present study an attempt was made to isolate a novel psychrophilic bacterial strain producing cold active alpha amylase. Among the 16 isolates observed on starch agar plate, six showed zone of hydrolysis on treatment with Gram’s iodine. Out of these 6 isolates, isolate KS-5 gave maximum alpha amylase production. During optimization of production conditions, 6 days old inoculum at a concentration of 0.5% gave maximum production of α-amylase when the inoculated production medium was incubated for 96 h. The isolate gave maximum production of α-amylase in medium 5 with starch as carbon source at a concentration of 9%. Approximately 2-fold increase in activity was observed after the optimization of above parameters, further on complete optimization of production conditions, a great increase in amylase production can be achieved. The properties of this cold active α-amylase suggest its usefulness in commercial sectors. 

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

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FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative