Graphene and Zinc Oxide nanoparticles (ZnO-NPs) have unique properties in the diagnosis and treatment of diseases. By loading ZnO-NPs on graphene, their biological compatibility can be greatly improved. In the present study, we investigated the anti-angiogenesis properties of ZnO-NPs synthesized using Crocus Sativus petal extract loaded on graphene (CS/ZnO-NPs/G) on chick Chorioallantoic Membrane (CAM). The results showed that CS/ZnO-NPs/G reduced significantly the number and length of blood vessels, crown roost length and weight in chick embryo chorioalantoic membrance compared to control. The findings suggest that CS/ZnO-NPs have inhibitory effect on angiogenesis and can be promising to treat many diseases, such as cancer and chemotherapy.
