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2016 Volume 7 Issue 3

Study of cross linked enzyme aggregate of glucose isomerase of Streptomyces thermonitrificans immoblised on magnetic particle


Arun Gupta, S. K. Srivastava
Abstract
Magnetic cross linked enzyme aggregate of glucose isomerase were prepared by chemical cross-linking of enzyme aggregates with amino functionalized magnetic particle. The result of X-Ray Diffraction (XRD) has shown that the particles are in hematite phase (Fe2O3) and 47.84nm in size. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the existence of the surface coating. Scanning probe microscopy (SPM) was used to confirm the presence of protein on the particle surface. The magnetic CLEAs displayed a shift in optimal pH towards the acidic side, whereas optimal temperature of magnetic CLEAs was shifted to higher temperature, making conditions favorable for commercial use. Moreover, the magnetic CLEAs retained 80% initial activity even after 6 cycles of reuse.

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative