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2014 Volume 5 Issue 2

Scale up production of Protease using Pseudomonas aeruginosa MCM B-327 and its Detergent Compatibility


Abstract
The maximum protease activity was obtained from P. aeruginosa MCM B-327 with soybean meal 1%, tryptone 1%, initial medium pH 7, agitation rate 250 rpm, aeration rate 0.75 vvm and fermentation temperature 30 °C, under submerged fermentation conditions (SmF). The protease productivity at 10 and 120L fermenters was found to be 16,021 and 9,975 UL-1h-1 respectively. Kinetics of cell growth revealed that specific cell growth rate was 0.025 h-1. Protease was active and stable at different pH, temperatures, in anionic, cationic and non-ionic detergent additives, as well as in commercial detergents. The protease exhibited blood stains removing performance indicating its potential in detergent industry. The dried ammonium sulphate precipitated protease was stable at room temperature for a period of one year. The Protease has shown properties suitable for its application in detergents. The results contribute to basic knowledge and application of protease from P.aeruginosa to detergent industry. The studies will help to optimize the production of this protease for biotechnological applications.

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative