Microbial fuel cell was investigated for electricity generation with secondary treated waste water as a filling material and a source for organic matter. Different electrodes were tested; Aluminum-Graphite (AL-C), Iron –Graphite (Fe-C) and Copper- Graphite (Cu-C).The electricity generation of MFC was tested when enriched with different concentrations of sodium acetate (400, 600 and 800mg/l acetate). Maximum power density reached 816mW/m2 for double MFC using proton exchange membrane (PEM) with the corresponding power density of 880mW/m2 for single MFC without using PEM with 800mg/l acetate with the (AL-C). Relation between electrode spacing and power density was studied and found that the least electrode spacing (1 cm) producing the highest power density 797.3mW/m2 for single cell and (803.51mw/m2) for double cell. Further research is needed to maximize the cell for possible application to new settlements in Sinai to provide energy and minimize underground contamination by wastewater.