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2010 Volume 2 Issue 4

Characterizing and improving the thermostability of purified xylanase from Aspergillus niger DFR-5 grown on solid-state-medium


Abstract
 The thermostability of absolutely purified xylanase from Aspergillus niger DFR-5 was improved using polyols. Supplementation of sorbitol at 2M concentration was found to increase the half-life and D-value of xylanase at elevated temperatures (45-70ºC). Thermodynamic parameters associated with the process were analyzed revealing that the stability at higher temperatures was due to the increased enthalpy (∆Hº) and free energy (∆Gº) change of enzyme denaturation in the presence of sorbitol. The negative values of ∆Sº (-150.093 Jmol-1K-1 at 70ºC) clearly indicated that enzyme underwent a significant process of aggregation during denaturation. The enzyme required divalent cations for maximum activity and inhibited by chelator. The diminution of activity by various thiol-binding agents and enhancement by reducing agents like β-ME confirmed the essentiality of cysteine for catalysis. The enzyme had a half-life and D-value of 277 and 921 days when stored at 4 ºC.

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative