Diabetic retinopathy, the most common diabetic eye disease, is caused by changes in the blood vessels of the retina which remains the major cause. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. One of the biomarker for Diabetic retinopathy has been identified as Vascular Endothelial Growth Factor ( VEGF )gene by computational analysis. VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signalling proteins involved in both vasculogenesis and angiogenesis, Over expression of VEGF can cause vascular disease in the retina of the eye and other parts of the body. Drugs can inhibit VEGF and control or slowdown those disease. Computational analysis of VEGF with other genes responsible for diabetic retinopathy were done by aligning those genes by pair wise and multiple sequence alignments. MSA shows VEGF’s role in diabetic retinopathy and its related with other genes and proteins responsible for pathogenesis of diabetic retinopathy. Also the determination of the promoter and conserved domain of VEGF gene help us to identify its expression levels. Thus molecular docking studies were carried out to analyse the biomarker VEGF, that helps in treatment of diabetic retinopathy which is proliferative in nature due to uncontrolled angiogenesis.