Effect of hormones, explants and genotypes in in vitro culturing of sorghum

J Biochem Tech

Volume 1, Issue 4, August 2009, Page No. 96-103

Effect of hormones, explants and genotypes in in vitro culturing of sorghum

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Indra Arulselvi P

, Krishnaveni S

Department of Biotechnology, Periyar University, Salem, Tamil
Nadu, India, Pin: 636011

Received: 12 March 2009 / Received in revised form: 18 July 2009, Accepted: 5 August 2009, Published online: 21 September 2009

Abstract

In vitro culturing of sorghum is very much influenced by genotypes,
explants, media composition and hormones. Among the genotypes
tested CO 25 was found to show high frequency of callus induction
and regeneration. Regeneration efficiency of embryogenic calli from
CO 25 was found to be greater (88-98%) than that of calli derived
from other explants (Immature inflorescence, Mature embryo and
Shoot tips) of different genotypes (CO 26, TNS 586 and CO-S-28).
Out of the different media tried for callus induction and regeneration
of sorghum, I6 medium + kinetin 0.5 mg l-1 + 2,4-D 2 mg l-1 showed
high frequency of embryogenic calli from immature embryo of
CO25. Regeneration medium I6 + Kinetin (1.0) + NAA (0.5) +
casein hydrolysate at levels of 250 mg l-1 and 500 mg l-1 was found
to best medium composition for regeneration. An efficient protocol
has been developed from suitable explant and genotype for callus
induction, regeneration of sorghum and thus to produce transgenic
plants for biotic and abiotic resistance.

Keywords: In vitro culture, Sorghum, explants, genotypes, hormones

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J Biochem Tech

Volume 1, Issue 4, August 2009, Page No. 96-103




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